TagMaster Site-Directed Mutagenesis Kit, 6 rxn

It is the first kit of its kind that can insert or switch tags (such as 6xHis, Myc, Flag, HA, V5) into a gene in a simple, single reaction. It also allows for easy construction of CRISPR/Cas9 expression plasmids by switching or inserting guide RNA sequences in one reaction.

Key Features

• Powerful & Versatile
  • Insertion/substitution (1-60 bp)
  • Deletion (1-660 bp)
  • Multi-site mutations (2 sites)
  • Insert/switch tags (6xHis, Myc, Flag, HA, V5, etc.)
  • Insert/switch sgRNA sequences in CRISPR/Cas9 plasmids
  • Suitable for plasmids up to 10 kb
• Efficient: Achieve 80% to >99% mutation rates.
• Accurate: A high-fidelity enzyme blend and a linear DNA amplification (non-PCR) procedure ensure accuracy.
• Fast and Simple: An easy 2-step protocol with a 1-2 hour reaction time.